Our antibodies are guaranteed and products are delivered with comprehensive analytical data.

• Rabbit and Guinea Pig polyclonal antibodies against Peptides and Proteins.
• Standard Protocol:
• 40 days, 2 rabbits for a total of 100 ml of ELISA-tittered antiserum.
• 34 days, 2 guinea pigs for a total of 20 ml of ELISA-tittered antiserum.

Full characterization and report: SDS-PAGE, ELISA, and/or WESTERN BLOT

1- Anti-Peptide Polyclonal Antibodies
This service includes the following features:

• Antigen preparation:

• 20 mg of peptide (lyophilized or solubilized (> 10 mg/ml)) should be provided for the immunization of 2 rabbits (or 12 mg of peptide for the immunization of 2 guinea pigs).
• Peptide should be at least 10 residues long and include a Cys (N- or C-terminal) for KLH conjugation purposes.
• Standard antigen is prepared by covalently linking your Peptide to KLH carrier protein.
  

• Immunization of 2 animals (2 rabbits or 2 guinea pigs) for:

• 100 ml of rabbit antiserum tittered by ELISA (or 20 ml of guinea pig antiserum).
• 5 ml of rabbit pre-immune serum for control (or 1 ml of guinea pig pre-immune serum).

ELISA (DO at 405 nm) : Peptide X   Animal 1 Animal 2   1/1 000 1/10 000 1/100 000 AS-1 1,602 1,229 0,721 AS-2 1,638 1,312 0,767 PI1 0,153 0,047 0,032 PI2 0,146 0,041 0,038         AS : Antiserum PI : Pre-immune Serum 1/1 000 à 1/100 000 : Dilutions

2- Anti-Protein Polyclonal Antibodies
This service includes the following features:

• Antigen preparation:

• 5 mg of protein (lyophilized or solubilized (> 2.5 mg/ml)) should be provided for the immunization of 2 rabbits (or 3 mg of protein for the immunization of 2 guinea pigs).
• Protein should be over 10kDa in size to ensure good immunization. 
• Smaller proteins would require conjugation to a larger protein carrier (e.g. KLH).

• Immunization of 2 animals (2 rabbits or 2 guinea pigs) for:

• 100 ml of rabbit antiserum tittered by ELISA (or 20 ml of guinea pig antiserum).
• 5 ml of rabbit pre-immune serum for control (or 1 ml of guinea pig pre-immune serum).
• SDS-PAGE & WESTERN BLOT analysis.

Example of results:

ELISA (DO at 405 nm) : Protéine Y   Animal 1 Animal 2   1/1 000 1/10 000 1/100 000 AS-1 1,711 1,313 0,795 AS-2 1,740 1,302 0,849 PI1 0,153 0,041 0,033 PI2 0,175 0,049 0,036  AS : Antiserum   PI : Pre-immune Serum   1/1 000 à 1/100 000 : Dilutions

SDS-PAGE at Western Blot

3- Affinity Purification
Following polyclonal antibodies production as described above, you may wish to purify your antiserum by affinity chromatography.

This service includes:

• Preparation of the affinity chromatographic support by covalently linking of your peptide or protein.
• Affinity chromatography of your antiserum.
• Analysis of collected fractions.

From 100 ml of serum, we will deliver approximately 38 ml of purified, ELISA-tittered antiserum (~ 1mg/ml).

Example of results:

Antibodies purification by affinity chromatography

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This service includes the immunization and two affinity purification steps. Deliverables include:

• Phospho-specific antiserum fraction.
• Antiserum fraction specific to the non-phosphorylated sequence.

Exemple of results :

ELISA (DO at 405 nm) :

PI : Pre-Immune Serum
AS : Crude Antiserum
PP : Phospho-specific Fraction
nPP : NON-phospho-specific Fraction

5- Total IgG purification
We also propose the purification of total IgG containing in your antiserum using a simple, rapid, and effective protocol:

• Proteinic precipitation.
• Ion exchange chromatography.

From 100 ml of serum, we will deliver approximately 50 ml of purified, ELISA-tittered IgG (~ 9 mg/ml).
Exemple of results :

SDS-PAGE of purified-IgG fractions

Contact:
Please send your sample clearly marked and wrapped with parafilm in a padded envelope. Include a letter describing your samples (mass amount, solution content, concentration if applicable).

Please send it using express delivery courier or postal services at :