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POLYCLONAL ANTIBODIES

Our antibodies are guaranteed and products are delivered with comprehensive analytical data.

  • Rabbit and Guinea Pig polyclonal antibodies against Peptides and Proteins.
  • Standard Protocol:
    • 40 days, 2 rabbits for a total of 100 ml of ELISA-tittered antiserum.
    • 34 days, 2 guinea pigs for a total of 20 ml of ELISA-tittered antiserum.

Full characterization and report: SDS-PAGE, ELISA, and/or WESTERN BLOT

1- Anti-Peptide Polyclonal Antibodies
This service includes the following features:

  • Antigen preparation:
    • 20 mg of peptide (lyophilized or solubilized (> 10 mg/ml)) should be provided for the immunization of 2 rabbits (or 12 mg of peptide for the immunization of 2 guinea pigs).
    • Peptide should be at least 10 residues long and include a Cys (N- or C-terminal) for KLH conjugation purposes.
    • Standard antigen is prepared by covalently linking your Peptide to KLH carrier protein.
  • Immunization of 2 animals (2 rabbits or 2 guinea pigs) for:
    • 100 ml of rabbit antiserum tittered by ELISA (or 20 ml of guinea pig antiserum).
    • 5 ml of rabbit pre-immune serum for control (or 1 ml of guinea pig pre-immune serum).

Example of results:

ELISA (DO at 405 nm) :


Peptide X

 

Animal 1

Animal 2

 

1/1 000

1/10 000

1/100 000

AS-1

1,602

1,229

0,721

AS-2

1,638

1,312

0,767

PI1

0,153

0,047

0,032

PI2

0,146

0,041

0,038

 

 

 

 

AS : Antiserum
PI : Pre-immune Serum
1/1 000 à 1/100 000 : Dilutions


2- Anti-Protein Polyclonal Antibodies
This service includes the following features:

  • Antigen preparation:
    • 5 mg of protein (lyophilized or solubilized (> 2.5 mg/ml)) should be provided for the immunization of 2 rabbits (or 3 mg of protein for the immunization of 2 guinea pigs).
    • Protein should be over 10kDa in size to ensure good immunization. 
    • Smaller proteins would require conjugation to a larger protein carrier (e.g. KLH).
  • Immunization of 2 animals (2 rabbits or 2 guinea pigs) for:
    • 100 ml of rabbit antiserum tittered by ELISA (or 20 ml of guinea pig antiserum).
    • 5 ml of rabbit pre-immune serum for control (or 1 ml of guinea pig pre-immune serum).
    • SDS-PAGE & WESTERN BLOT analysis.

Example of results:

ELISA (DO at 405 nm) :

Protéine Y

 

Animal 1

Animal 2

 

1/1 000

1/10 000

1/100 000

AS-1

1,711

1,313

0,795

AS-2

1,740

1,302

0,849

PI1

0,153

0,041

0,033

PI2

0,175

0,049

0,036

 AS : Antiserum
  PI : Pre-immune Serum
  1/1 000 à 1/100 000 : Dilutions


SDS-PAGE at Western Blot

3- Affinity Purification
Following polyclonal antibodies production as described above, you may wish to purify your antiserum by affinity chromatography.

This service includes:

    • Preparation of the affinity chromatographic support by covalently linking of your peptide or protein.
    • Affinity chromatography of your antiserum.
    • Analysis of collected fractions.

From 100 ml of serum, we will deliver approximately 38 ml of purified, ELISA-tittered antiserum (~ 1mg/ml).

Example of results:


Antibodies purification by affinity chromatography

.

4- Phospho-Specific Antibodies (peptide)

This service includes the immunization and two affinity purification steps. Deliverables include:

    • Phospho-specific antiserum fraction.
    • Antiserum fraction specific to the non-phosphorylated sequence.

Exemple of results :

ELISA (DO at 405 nm) :

 

Antigen : Peptide X (p)

Antigen : Peptide X

Dilution

PI

AS

PP

nPP

PI

AS

PP

nPP

1/1K

0,153

1,616

1,772

1,163

0,161

1,464

0,298

1,582

1/2K

0,111

1,481

1,636

1,062

0,122

1,340

0,286

1,431

1/4K

0,078

1,347

1,548

0,974

0,083

1,227

0,275

1,337

1/8K

0,055

1,253

1,465

0,913

0,058

1,115

0,259

1,219

1/16K

0,040

1,092

1,213

0,782

0,044

0,985

0,211

1,083

1/32K

0,032

0,962

1,071

0,685

0,032

0,868

0,185

0,936

1/64K

0,028

0,831

0,905

0,596

0,029

0,749

0,168

0,825

1/128K

0,025

0,745

0,812

0,518

0,027

0,665

0,146

0,724

PI : Pre-Immune Serum
AS : Crude Antiserum
PP : Phospho-specific Fraction
nPP : NON-phospho-specific Fraction

 


 

 

 

 

 

5- Total IgG purification
We also propose the purification of total IgG containing in your antiserum using a simple, rapid, and effective protocol:

      • Proteinic precipitation.
      • Ion exchange chromatography.

From 100 ml of serum, we will deliver approximately 50 ml of purified, ELISA-tittered IgG (~ 9 mg/ml).
Exemple of results :


SDS-PAGE of purified-IgG fractions

Contact:
Please send your sample clearly marked and wrapped with parafilm in a padded envelope. Include a letter describing your samples (mass amount, solution content, concentration if applicable).

Please send it using express delivery courier or postal services at :

Pr. Abdelaziz SOUKRI
Laboratoire de Physiologie et de Génétique Moléculaire (PGM)
Faculté des Sciences Aïn Chock
Email : LaboPGM@fsac.ac.ma
Adresse : Km 8, ROUTE D’EL JADIDA B.P 5366 MAARIF
CASABLANCA 20100 MAROC
Téléphone: (212) (0) 63 09 66 34
Fax: (212) (0) 22 23 06 74

 
© 2006 Faculté des Sciences Ain Chock Casablanca Maroc